1YGE

LIPOXYGENASE-1 (SOYBEAN) AT 100K

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1993-04
Detector	MAC Science DIP-2000
Spacegroup name	P 1 21 1
Unit cell lengths	94.900, 94.000, 49.900
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	10.000 - 1.400
R-factor	0.197
Rwork	0.197
R-free	0.24300
RMSD bond length	0.011 *
RMSD bond angle	1.700 *
Data reduction software	HKL ((DENZO))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	1.400 *	1.400 *
Rmerge	0.067
Number of reflections	161029
Completeness [%]	96.0	92 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	acetate	0.1 (M)
2	1	drop	PEG3400	4 (%(w/v))
3	1	drop	enzyme	4-5 (mg/ml)
4	1	reservoir	PEG3400	8 (%(w/v))