1YFU
Crystal structure of 3-hydroxyanthranilate-3,4-dioxygenase from Ralstonia metallidurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 58.100, 58.100, 230.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.110 - 1.900 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.24000 |
Structure solution method | SAD + MR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.110 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 17369 | |
Completeness [%] | 90.3 | 72.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 8000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 8000, magnesium chloride, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |