1YFS

The crystal structure of alanyl-tRNA synthetase in complex with L-alanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	173.026, 73.868, 73.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.080
R-factor	0.2
Rwork	0.189
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1riq
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.080
Number of reflections	57744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	PEG 5000 mme, NaCl, Tris-Cl, Tris-base, L-alanine, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K