1YFQ
High resolution S. cerevisiae Bub3 mitotic checkpoint protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.274, 74.089, 94.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.100 |
R-factor | 0.152 |
Rwork | 0.152 |
R-free | 0.18600 |
Structure solution method | MIR |
RMSD bond length | 0.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.130 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.043 | 0.422 |
Number of reflections | 137806 | |
<I/σ(I)> | 18.7 | 3.05 |
Completeness [%] | 97.8 | 91.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 8000, Ca acetate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K |