1YFN
Versatile modes of peptide recognition by the AAA+ adaptor protein SspB- the crystal structure of a SspB-RseA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.647, 60.533, 88.576 |
Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
Resolution | 29.430 - 1.800 |
R-factor | 0.241 |
Rwork | 0.234 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1OU8 dimer with peptide removed |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 57718 | |
Completeness [%] | 94.0 | 99 |
Redundancy | 6.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.2 M Sodium Formate, 10 mM Magnesium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |