1YFM
RECOMBINANT YEAST FUMARASE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 287 |
Detector technology | AREA DETECTOR |
Collection date | 1994-03 |
Detector | SIEMENS |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 95.190, 95.190, 100.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.31500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fuo |
RMSD bond length | 0.014 |
RMSD bond angle | 21.940 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.620 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.109 | |
Total number of observations | 118256 * | |
Number of reflections | 14127 | |
<I/σ(I)> | 6.9 | 0.487 |
Completeness [%] | 94.5 | 66.7 * |
Redundancy | 7.9 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.3 | 150 MM TRIS-HCL PH 8.3, 100 MM LI2SO4, 8-10% PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Tris-HCl | 150 (mM) | |
2 | 1 | 1 | 100 (mM) | ||
3 | 1 | 1 | PEG4000 | 8-10 (%) |