1YFI
Crystal Structure of restriction endonuclease MspI in complex with its cognate DNA in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-08 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 0.9040 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.295, 111.266, 130.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.940 - 2.700 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.282 |
| Number of reflections | 21426 | |
| <I/σ(I)> | 28.7 | 7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | MES, ammonium sulfate, calcium chloride, PEG 8000, glycerol , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | PEG 8000 | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | water | ||
| 6 | 1 | 2 | MES | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | PEG 8000 | ||
| 9 | 1 | 2 | glycerol |
