1YF5
Cyto-Epsl: The Cytoplasmic Domain Of Epsl, An Inner Membrane Component Of The Type II Secretion System Of Vibrio Cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97951 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.006, 88.707, 55.371 |
Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.750 |
R-factor | 0.21553 |
Rwork | 0.213 |
R-free | 0.26881 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w97 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.038 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.080 | 0.370 |
Number of reflections | 7122 | |
<I/σ(I)> | 15.1 | 3.4 |
Completeness [%] | 95.8 | 81 |
Redundancy | 7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG 6000, 150mM calcium acetate, 100mM tris pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |