1YF4

Crystal Structure of trypsin-vasopressin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	300
Detector technology	AREA DETECTOR
Collection date	2003-10-07
Detector	ENRAF-NONIUS
Spacegroup name	P 21 21 21
Unit cell lengths	59.274, 61.953, 69.146
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.980
R-factor	0.185
Rwork	0.185
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Porcine beta trrypsin
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.030
High resolution limit [Å]	1.980	1.980
Rmerge	0.087
Number of reflections	14352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	293	acetate buffer, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K