1YF2
Three-dimensional structure of DNA sequence specificity (S) subunit of a type I restriction-modification enzyme and its functional implications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.972, 94.222, 103.520 |
Unit cell angles | 90.00, 95.01, 90.00 |
Refinement procedure
Resolution | 48.520 - 2.400 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.27900 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 59867 | |
Completeness [%] | 26.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG 3000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |