1YE3
HORSE LIVER ALCOHOL DEHYDROGENASE APOENZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.780, 74.290, 181.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.200 - 1.590 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.25593 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8adh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.529 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.200 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.075 | 0.211 |
| Number of reflections | 36732 | |
| <I/σ(I)> | 11.5 | 4.7 |
| Completeness [%] | 71.9 | 58 |
| Redundancy | 5.66 | 5.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8.4 | 278 | 50 mM Tris-HCl, 25% methylpentanediol, pH 8.4, MICRODIALYSIS, temperature 278K |
