1YDM
X-Ray structure of Northeast Structural Genomics target SR44
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97859,0.97897,0.97243 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 85.302, 44.276, 85.296 |
Unit cell angles | 90.00, 104.99, 90.00 |
Refinement procedure
Resolution | 28.350 - 2.500 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.30400 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.061 | 0.419 |
Number of reflections | 18301 | |
<I/σ(I)> | 12.5 | 2.1 |
Completeness [%] | 75.5 | |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 26% PEG8000, 50 mM Tris, 0.2M AmSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |