1YDH

X-ray structure of a lysine decarboxylase-like protein from arabidopsis thaliana gene at5g11950

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-15
Detector	APS-1
Spacegroup name	C 1 2 1
Unit cell lengths	121.589, 80.427, 50.709
Unit cell angles	90.00, 102.97, 90.00

Refinement procedure

Resolution	35.450 - 2.152
R-factor	0.16164
Rwork	0.159
R-free	0.21300
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.939
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.451	50.000	2.230
High resolution limit [Å]	2.152	4.740	2.150
Rmerge	0.384	0.119	0.384
Total number of observations		2537	2460
Number of reflections	25771
<I/σ(I)>	6.266		2.401
Completeness [%]	99.9	99.6	100
Redundancy	6.9		6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	293	10 MG/ML PROTEIN, 13% MPEG 2K, 0.28 M POTASSIUM NITRATE, 0.1 M MOPS, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.00