1YD7
Conserved hypothetical protein Pfu-1647980-001 from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 61.448, 61.448, 129.084 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.890 - 2.300 |
R-factor | 0.20764 |
Rwork | 0.206 |
R-free | 0.24274 |
Structure solution method | SAS |
RMSD bond length | 0.015 |
RMSD bond angle | 1.450 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.080 | 0.058 | 0.477 |
Number of reflections | 17709 | ||
Completeness [%] | 100 | 19.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | modified microbatch | 8.9 | 291 | 0.1M TRIS-HCl/NaOH, 0.5M ammonium sulfate, pH 8.9, modified microbatch, temperature 291K |