1YCS
P53-53BP2 COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Collection date | 1995-12-22 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.800, 72.800, 119.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.28600 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.640 |
| Data reduction software | HKL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.057 |
| Total number of observations | 220654 * |
| Number of reflections | 30230 |
| Completeness [%] | 99.1 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | BTP | 50 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 10 (%) | |
| 6 | 1 | reservoir | ethylene glycol | 8 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | 300 (mM) | ||
| 9 | 1 | reservoir | dithiothreitol | 10 (mM) |
