1YCR

MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF P53

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-07-25
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	43.414, 100.546, 54.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.2
Rwork	0.200
R-free	0.27600
RMSD bond length	0.013
RMSD bond angle	1.550
Data reduction software	HKL
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.052
Total number of observations	16069 *
Number of reflections	3559
Completeness [%]	90.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MDM2	1.5 (mM)
2	1	drop	MES	25 (mM)
3	1	drop		250 (mM)
4	1	drop	DTT	5 (mM)
5	1	reservoir	potassium phosphate	79 (%sat)
6	1	reservoir	Tris-HCl	50 (mM)
7	1	reservoir	DTT	5 (mM)