1YBZ

Conserved hypothetical protein from Pyrococcus furiosus Pfu-1581948-001

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0090
Spacegroup name	P 43 21 2
Unit cell lengths	52.501, 52.501, 80.217
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.937 - 1.820
R-factor	0.20257
Rwork	0.201
R-free	0.23660
Structure solution method	SAS
RMSD bond length	0.013
RMSD bond angle	1.238
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.890
High resolution limit [Å]	1.820	3.920	1.820
Rmerge	0.055	0.039	0.231
Total number of observations		1182	978
Number of reflections	10567
Completeness [%]	99.4	99.7	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	modified microbatch	7.5	291	0.8M sodium dihydrogen phosphate, 0.8M disodium hydrogen phosphate, 0.1M HEPES, pH 7.5, modified microbatch, temperature 291K