1YBM
X-ray structure of selenomethionyl gene product from Arabidopsis thaliana at5g02240 in space group P21212
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97935, 0.97904, 0.96389 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.634, 77.286, 92.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.090 - 2.096 |
Rwork | 0.189 |
R-free | 0.26150 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.643 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.087 | 50.000 | 2.150 |
High resolution limit [Å] | 2.096 | 5.180 | 2.100 |
Rmerge | 0.047 | 0.033 | 0.280 |
Total number of observations | 1967 | 1878 | |
Number of reflections | 29937 | ||
<I/σ(I)> | 22.745 | 6.125 | |
Completeness [%] | 93.0 | 84.8 | 88.3 |
Redundancy | 11.7 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 MG/ML PROTEIN, 24 PERCENT PEG 4K, 0.136 M SODIUM MALONATE, 0.10 M BISTRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |