1YB6
Hydroxynitrile lyase from hevea brasiliensis in complex with mandelonitrile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8426 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.362, 106.553, 128.351 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.680 - 1.540 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.18200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2yas |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.680 | 1.580 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 46599 | |
| <I/σ(I)> | 28.1 | 7.6 |
| Completeness [%] | 95.8 | 96 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | AMMONIUM SULFATE, PEG 400, pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
