1YB5

Crystal structure of human Zeta-Crystallin with bound NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-09-23
Detector	RIGAKU RAXIS HTC
Spacegroup name	P 21 21 2
Unit cell lengths	99.484, 110.239, 77.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.150 - 1.850
R-factor	0.17062
Rwork	0.170
R-free	0.20667
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.572
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.360	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.084	0.578
Number of reflections	73050
<I/σ(I)>	11.5	2
Completeness [%]	99.8	99.5
Redundancy	3.5	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	14.4% PEG 10K, 0.16 calcium acetate, 20% glycerol, 0.08 M cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K