1YB3
Conserved hypothetical protein Pfu-178653-001 from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.134, 44.388, 55.326 |
Unit cell angles | 90.00, 118.82, 90.00 |
Refinement procedure
Resolution | 48.450 - 1.600 |
R-factor | 0.20521 |
Rwork | 0.204 |
R-free | 0.22910 |
Structure solution method | SAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.151 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.052 | 0.042 | 0.218 |
Total number of observations | 3129 | 1066 | |
Number of reflections | 26254 | ||
Completeness [%] | 84.8 | 98.2 | 34.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | modified microbatch | 3.6 | 291 | 12% w/v PEG 4000, 0.2M sodium chloride, 0.2M magnesium chloride, 0.1M sodium citrate, pH 3.6, modified microbatch, temperature 291K |