1YB1

Crystal structure of human 17-beta-hydroxysteroid dehydrogenase type XI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-10-20
Detector	RIGAKU RAXIS HTC
Spacegroup name	P 41 21 2
Unit cell lengths	110.939, 110.939, 118.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.000 - 1.950
R-factor	0.16254
Rwork	0.162
R-free	0.19212
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MR MODEL OBTAINED BY AUTOBUILDING INTO A SELENOMETHIONINE SAD PHASED MAP
RMSD bond length	0.010
RMSD bond angle	1.344
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.091
Number of reflections	61906
Completeness [%]	100.0	100
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.08M Sodium Citrate pH5, 2.52M Ammonium Sulphate , VAPOR DIFFUSION, SITTING DROP, temperature 293K