1YAZ
AZIDE-BOUND YEAST CU(II)/ZN SUPEROXIDE DISMUTASE ROOM TEMPERATURE (298K) STRUCTURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-15 |
| Detector | MACSCIENCE |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 119.375, 119.375, 74.543 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 90.000 - 1.700 |
| R-factor | 0.169 |
| R-free | 0.20800 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1JCW |
| RMSD bond length | 0.020 |
| RMSD bond angle | 27.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.097 * | 0.420 * |
| Total number of observations | 133918 * | |
| Number of reflections | 22142 | |
| <I/σ(I)> | 17.1 | 2 |
| Completeness [%] | 98.5 | 89.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.7 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-25 (mg/ml) | |
| 2 | 1 | drop | phosphate | 50 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0-2.2 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | 0.01 (%(w/v)) |
