1YAX
Cystal structure Analysis of S.typhimurium PhoQ sensor domain with Calcium
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2003-11-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.078, 134.871, 58.444 |
Unit cell angles | 90.00, 119.61, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.244 |
Rwork | 0.242 |
R-free | 0.27952 |
Structure solution method | SAD and MR |
RMSD bond length | 0.013 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.040 | 0.253 |
Number of reflections | 29394 | |
<I/σ(I)> | 35.29 | 3.68 |
Completeness [%] | 95.0 | 72.2 |
Redundancy | 3.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8000, tris-HCL, calcium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 8000, tris-HCL, calcium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |