1YAV
Crystal structure of CBS domain-containing protein ykuL from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-12 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.564, 69.592, 70.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.410 - 2.100 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26200 |
| Structure solution method | Sulfur SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 17293 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 95.1 | 67.6 |
| Redundancy | 41.7 | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Ammonium sulfate, Hepes, Xylitol, KCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
