1YAU
Structure of Archeabacterial 20S proteasome- PA26 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 255.224, 126.913, 181.027 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.18038 |
| Rwork | 0.180 |
| R-free | 0.23292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pma |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.381 |
| Number of reflections | 224164 | |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 273 | 0.1M Na-citrate/phosphate buffer, 0.2M Lithium Sulfate, 15% PEG-1000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
