1YAR
Structure of Archeabacterial 20S proteasome mutant D9S- PA26 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.283 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 254.874, 127.512, 181.182 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 47.300 - 1.900 |
| R-factor | 0.18196 |
| Rwork | 0.182 |
| R-free | 0.21624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pma |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.271 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.497 |
| Number of reflections | 442007 | |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 97.4 | 95.8 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 273 | 0.1M Na-citrate/phosphate buffer, 0.2M Lithium Sulfate, 15% PEG-1000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
