1YAB

Structure of T. maritima FliN flagellar rotor protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-01-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979277
Spacegroup name	P 31 2 1
Unit cell lengths	100.173, 100.173, 87.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 3.400
R-factor	0.22735
Rwork	0.224
R-free	0.28624
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1O6A.pdb
RMSD bond length	0.014
RMSD bond angle	1.760
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.700	3.520
High resolution limit [Å]	3.400	3.400
Number of reflections	7246
<I/σ(I)>	7.3	1.8
Completeness [%]	95.9	93.4
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	293	18% MPD, 100mM MES buffer, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K