1YAA
ASPARTATE AMINOTRANSFERASE FROM SACCHAROMYCES CEREVISIAE CYTOPLASM
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-10 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 130.310, 134.630, 98.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.050 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cst |
| RMSD bond length | 0.017 |
| RMSD bond angle | 23.000 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.140 | |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.120 | |
| Total number of observations | 470081 * | |
| Number of reflections | 100347 * | |
| Completeness [%] | 93.0 | 88 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 * | Jeffery, C.J., (1998) Acta Cryst., D54, 659. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-15 (mg/ml) | |
| 2 | 1 | drop | PMSF | 0.010 (mM) | |
| 3 | 1 | drop | PLP | 0.010 (mM) | |
| 4 | 1 | drop | sodium acetate | 10 (mM) | |
| 5 | 1 | drop | sodium maleate | 0.100 (mM) | |
| 6 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | acetate | 0.1 (M) | |
| 8 | 1 | reservoir | PEG4000 | 22-20 (%) |
