1Y9W
Structural Genomics, 1.9A crystal structure of an acetyltransferase from Bacillus cereus ATCC 14579
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-18 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9797,0.94656 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.032, 65.344, 89.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.800 - 1.900 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.558 |
| Number of reflections | 45795 | |
| <I/σ(I)> | 30.4 | 2.18 |
| Completeness [%] | 93.4 | 85.2 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.05M MgCl2, 0.1M HEPES, 30% PEG MME550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
