1Y9K
IAA acetyltransferase from Bacillus cereus ATCC 14579
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2004-07-15 |
Detector | SBC-2 |
Wavelength(s) | 0.97945 |
Spacegroup name | P 32 |
Unit cell lengths | 70.122, 70.122, 171.516 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.400 - 2.390 |
R-factor | 0.20029 |
Rwork | 0.198 |
R-free | 0.23464 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.400 | 2.390 |
High resolution limit [Å] | 2.390 | 2.390 |
Number of reflections | 37374 | |
<I/σ(I)> | 19.85 | |
Completeness [%] | 99.5 | 94.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 2.0 M Ammonium sulfate, CAPS pH 10.5 0.1 Lithium Sulfate, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |