1Y9I
Crystal structure of low temperature requirement C protein from Listeria monocytogenes
Experimental procedure
Experimental method | Dual Wavelength |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979, 0.94 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.908, 101.069, 63.096 |
Unit cell angles | 90.00, 95.40, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
Rwork | 0.211 |
R-free | 0.24500 |
Structure solution method | Dual Wavelength |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.780 | 1.780 |
Number of reflections | 64929 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 97.0 | 86.1 |
Redundancy | 6.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 295 | Ammonium sulfate, pentaerythritol propoxylate, Bis-Tris, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 295K |