1Y9E
Crystal structure of Bacillus subtilis protein yhfP with NAD bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.271, 136.922, 167.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.800 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.23300 |
| Structure solution method | SAD |
| RMSD bond length | 0.046 |
| RMSD bond angle | 3.900 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.092 | 0.248 |
| Number of reflections | 130776 | |
| Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 4% PEG4000, 0.1M Bis-Tris pH 6.5, 0.16M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
