1Y9D
Pyruvate Oxidase variant V265A from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-11-08 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8123 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.660, 155.780, 100.750 |
Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
Resolution | 27.300 - 2.200 |
R-factor | 0.18068 |
Rwork | 0.178 |
R-free | 0.23794 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pow |
RMSD bond length | 0.018 |
RMSD bond angle | 1.677 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.300 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.062 |
Number of reflections | 147394 |
<I/σ(I)> | 20.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |