1Y9D
Pyruvate Oxidase variant V265A from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.660, 155.780, 100.750 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 27.300 - 2.200 |
| R-factor | 0.18068 |
| Rwork | 0.178 |
| R-free | 0.23794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pow |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.677 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.300 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.062 |
| Number of reflections | 147394 |
| <I/σ(I)> | 20.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
