1Y9D

Pyruvate Oxidase variant V265A from Lactobacillus plantarum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	110
Detector technology	CCD
Collection date	2003-11-08
Detector	MARRESEARCH
Wavelength(s)	0.8123
Spacegroup name	P 1 21 1
Unit cell lengths	94.660, 155.780, 100.750
Unit cell angles	90.00, 92.92, 90.00

Refinement procedure

Resolution	27.300 - 2.200
R-factor	0.18068
Rwork	0.178
R-free	0.23794
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pow
RMSD bond length	0.018
RMSD bond angle	1.677
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.300
High resolution limit [Å]	2.200
Rmerge	0.062
Number of reflections	147394
<I/σ(I)>	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1