1Y94
Crystal structure of the G16S/N17T/P19A/S20A/N67D Variant Of Bovine seminal Ribonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2004-01-01 |
Detector | ENRAF-NONIUS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.959, 61.102, 81.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.227 |
Rwork | 0.208 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r5d |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.057 | 0.246 |
Number of reflections | 12548 | |
<I/σ(I)> | 25.1 | 7 |
Completeness [%] | 98.2 | 99.5 |
Redundancy | 5.4 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000, TRIS-HCL, SODIUM ACETATE, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |