1Y8G

Catalytic and ubiqutin-associated domains of MARK2/PAR-1: Inactive double mutant with selenomethionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-14
Detector	MARRESEARCH
Wavelength(s)	0.8031
Spacegroup name	P 61
Unit cell lengths	118.822, 118.822, 105.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.300 - 2.501
R-factor	0.20136
Rwork	0.198
R-free	0.26834
Structure solution method	other
Starting model (for MR)	partially refined model of a similar construct obtained by combination of MR and MIR
RMSD bond length	0.016
RMSD bond angle	1.527
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	2.540
High resolution limit [Å]	2.501	2.501
Number of reflections	29259
<I/σ(I)>	17.9
Completeness [%]	99.9	100
Redundancy	7.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 5000 MME, TACSIMATE, BIS-TRIS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K