1Y82

Conserved hypothetical protein Pfu-367848-001 from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97623
Spacegroup name	P 21 21 21
Unit cell lengths	57.601, 103.667, 104.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.521 - 2.300
R-factor	0.22346
Rwork	0.222
R-free	0.27790
Structure solution method	SAS
RMSD bond length	0.017
RMSD bond angle	1.381
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.109	0.072	0.349
Total number of observations		3053	2788
Number of reflections	28211
Completeness [%]	98.7	99.9	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	modified microbatch	5.5	291	0.1M citrate, 0.1M sodium chloride, 0.1M lithium sulfate, 30% v/v PEG 400, pH 5.5, modified microbatch, temperature 291K
1	modified microbatch	5.5	291	0.1M citrate, 0.1M sodium chloride, 0.1M lithium sulfate, 30% v/v PEG 400, pH 5.5, modified microbatch, temperature 291K