1Y80
Structure of a corrinoid (factor IIIm)-binding protein from Moorella thermoacetica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.5798 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 55.689, 62.738, 34.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.630 - 1.700 |
R-factor | 0.1757 |
Rwork | 0.174 |
R-free | 0.21010 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.954 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.02) |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.077 | 0.064 | 0.216 |
Number of reflections | 13939 | ||
Completeness [%] | 89.3 | 38.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | modified microbatch | 6.7 | 291 | 0.1M Sodium HEPES, 1.6M ammonium sulfate, 30% 1,6-hexanediol, pH 6.7, modified microbatch, temperature 291K |