1Y7W
Crystal structure of a halotolerant carbonic anhydrase from Dunaliella salina
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418, 1.28255, 1.28295, 0.9840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.022, 119.835, 58.442 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 32.950 - 1.860 |
| R-factor | 0.194 |
| Rwork | 0.167 |
| R-free | 0.20200 |
| Structure solution method | MAD |
| Starting model (for MR) | 2cab |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 53083 | |
| <I/σ(I)> | 28 | |
| Completeness [%] | 98.4 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 2-propanol, PEG 4000, trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
