1Y79
Crystal Structure of the E.coli Dipeptidyl Carboxypeptidase Dcp in Complex with a Peptidic Inhibitor
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.050, 0.97880, 0.97910, 0.95000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.408, 67.921, 153.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.2403 |
Rwork | 0.214 |
R-free | 0.27470 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.370 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.341 |
Number of reflections | 45498 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.2 M potassium nitrate, 18% (w/v) PEG 3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |