1Y6L
Human ubiquitin conjugating enzyme E2E2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-22 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.109, 36.110, 130.568 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.19449 |
| Rwork | 0.192 |
| R-free | 0.24595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qcq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.588 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.260 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.044 | |
| Number of reflections | 39009 | |
| <I/σ(I)> | 23.28 | |
| Completeness [%] | 90.5 | 91.9 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 2M (NH4)2SO4, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
