1Y69
RRF domain I in complex with the 50S ribosomal subunit from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-14 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 168.700, 405.000, 693.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.130 - 3.330 |
R-factor | 0.275 |
Rwork | 0.275 |
R-free | 0.33800 |
Structure solution method | MR |
Starting model (for MR) | 1nkw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 343272 | |
<I/σ(I)> | 9.8 | 2.3 |
Completeness [%] | 95.2 | 86.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 290 | ETHANOL, DIMETHYLHEXANEDIOL, MGCL2, KCL, HEPES, NH4CL, pH 7.80, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ETHANOL | ||
10 | 1 | 2 | NH4CL | ||
2 | 1 | 1 | DIMETHYLHEXANEDIOL | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | KCL | ||
5 | 1 | 1 | HEPES | ||
6 | 1 | 1 | NH4CL | ||
7 | 1 | 1 | H2O | ||
8 | 1 | 2 | MGCL2 | ||
9 | 1 | 2 | KCL |