1Y56
Crystal structure of L-proline dehydrogenase from P.horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 172.219, 172.219, 175.398 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.850 - 2.860 |
| R-factor | 0.18396 |
| Rwork | 0.182 |
| R-free | 0.22557 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.564 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.410 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 37913 | |
| Completeness [%] | 98.7 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 10.5 | 298 | CAPS, NaH2PO4, K2HPO4, Li2SO4, pH 10.5, EVAPORATION, temperature 298K |
