1Y51
X-ray crystal structure of Bacillus stearothermophilus Histidine phosphocarrier protein (Hpr) F29W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-01 |
| Detector | MAC Science DIP-2030B |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 75.719, 75.719, 177.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.20742 |
| Rwork | 0.205 |
| R-free | 0.24653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild-type BstHpr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 31548 | |
| <I/σ(I)> | 34.7 | 2 |
| Completeness [%] | 98.4 | 90.5 |
| Redundancy | 9.6 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291 | 2M ammonium sulfate, 100mM phosphate-citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 291.0 K |
