1Y4Y
X-ray crystal structure of Bacillus stearothermophilus Histidine phosphocarrier protein (Hpr)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 74.830, 74.830, 179.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21401 |
| Rwork | 0.210 |
| R-free | 0.28269 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Swissmodel of BstHpr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.406 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17682 | |
| <I/σ(I)> | 17.1 | 3.2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | 2M ammonium sulfate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
