1Y4W
Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.954, 94.029, 67.634 |
Unit cell angles | 90.00, 107.04, 90.00 |
Refinement procedure
Resolution | 17.790 - 1.550 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.19820 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.168 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.790 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.045 | 0.150 |
Number of reflections | 78641 | |
<I/σ(I)> | 10.3 | 4.5 |
Completeness [%] | 91.0 | 85.7 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% PEG3350, 1mM sodium cacodylate, 20-100mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |