1Y4U
Conformation rearrangement of heat shock protein 90 upon ADP binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 158.008, 158.008, 117.022 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
Rwork | 0.240 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | a monomer of the HtpG-ADP complex |
RMSD bond length | 0.088 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.099 | 0.429 |
Number of reflections | 32234 | |
<I/σ(I)> | 14.9 | 2.1 |
Completeness [%] | 97.1 | 87.1 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.5 | 277 | 1.75 M ammonium sulfate, 0.1 M MES (pH 6.5), 3% DMSO and 1 mM NaN3, MICRODIALYSIS, temperature 277K |