1Y4M
Crystal structure of human endogenous retrovirus HERV-FRD envelope protein (syncitin-2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.95375 |
Spacegroup name | I 4 |
Unit cell lengths | 90.404, 90.404, 38.654 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.960 - 1.600 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mof |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.530 | 1.530 |
Number of reflections | 22623 | |
Completeness [%] | 94.9 | 75.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 292 | Sodium Chloride, Sodium Acetate tri-hydrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 292K |