1Y4H
Wild type staphopain-staphostatin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.948, 77.520, 84.891 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| R-factor | 0.18972 |
| Rwork | 0.188 |
| R-free | 0.21666 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pxv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.509 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.047 | 0.320 |
| Number of reflections | 53525 | |
| <I/σ(I)> | 30 | 3.3 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 294.15 | 2M (NH4)2SO4, 9% isopropanol , pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |
