1Y43
crystal structure of aspergilloglutamic peptidase from Aspergillus niger
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11-30 |
Detector | FUJI |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.100, 70.700, 38.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.400 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22600 |
Structure solution method | MIR |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.440 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 29424 | |
Completeness [%] | 97.5 | 82.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 2.1 | 298 | ammonium sulfate, dimethyl sulfoxide, glycine, pH 2.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |